Composite
Part:BBa_K575046:Design
Designed by: Kristin Palarz Group: iGEM11_Northwestern (2011-09-22)
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1282
Illegal NheI site found at 1305
Illegal NotI site found at 304 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 357
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2051
Illegal BsaI.rc site found at 1191
Design Notes
The constitutive promoter is located downstream of the inducible promoter because the reverse alignment may have resulted in unwanted leaky transcription of GFP.
Source
Except for the genomic promoter, the pieces of this construct are all existing registry parts.